Immunopharmacological and toxicological effect of ofloxacin in sheep

In this study, the effect of ofloxacin on the immune response was investigated in sheep vaccinated with the live attenuated sheep pox virus vaccine. The evaluation of the cellular immune response revealed a significant decrease in total leucocytic count, lymphocyte blastogenesis, phagocytosis% and bacterial killing% in groups administered 10 and 20 mg ofloxacin/kg b. wt. once daily for five successive days either simultaneously with sheep pox virus vaccine or one week post vaccination. The administration of ofloxacin in therapeutic [10 mg/kg b. wt.] and double therapeutic [20 mg/kg b. wt.] doses either simultaneously with sheep pox virus vaccine or one week post vaccination produced a significant reduction in antibody titers, total serum proteins, globulins and albumin. The biochemical assay of sera revealed a significant decrease in the level of packed cell volume, hemoglobin percentage and iron in ofloxacin treated groups either simultaneously with sheep pox virus vaccine or one week post vaccination. However, ofloxacin treatment five days prior to vaccination evoked a insignificant variation in cellular and humoral immune response

Some toxicological studies on croton oil in white rats

Eighty albino rats of both sexes were used in this study and divided into 4 equal groups. The first 3 groups were injected I/P with croton oil in a dose of 0.50 and 1.00 mg/k gm B. Wt. respectively while the 4th group was kept as control and injected 0.5 ml tween-80 I/P. Results revealed that R.B.Cs. count, HB% and PCV were decreased while W.B.Cs count were increased significantly. Differential leucocytic counts were significantly decreased in lymphocytes and significantly increased in neutrophils and monocytes while eosinophils were slightly increased but basophils were not affected. Levels of GOT, GPT and AP were significantly increased while T.P. albumin and globulin were significantly decreased in serum of toxicated rats. Urea and creatinine were decreased but potassium was increased in comparison to control group. Calcium and phosphorous levels were slightly changed in serum of treated rats. Microscopically the liver was congested with mononuclear aggregation and the hepatocyte suffered from degenerative changes. The kidney showed haemorrahages with haemosidrosis, mononuclear cell infilteration, congestive blood vessels with fibrinoid necrosis of their wall, the tubules showed degenerative changes and hyaline cast. The lung suffered from congetion with emphysema. The heart revealed haemorrahages with some hyalinization. The brain lesion was extravasated R.B.Cs. in meninges. Testicels showed few oedema in the intertubular spaces